National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Lipase production by lipolytic bacteria (Serratia marcescens), isolated from soil in Ohuhu Community in Umuahia North Local Government Area of Abia State, was investigated using a basal medium containing KH2PO4, Na2SO4, MgSO4.7H2O, Yeast extract, K2HPO4, Peptone, Glucose and Olive oil in a shake flask culture at 370C for 48hours. The study of different carbon sources on lipase production revealed that glucose yielded higher lipase production of 7.6Uml-1 than sucrose (6.2Uml-1), fructose (4.9Uml-1), starch (3.6Uml-1), lactose (2.4Uml-1) and maltose (1.4Uml-1). Subsequently, 2.5% concentration of glucose yielded higher lipase activity than the other concentrations (0.5%-4%) of glucose tested. Screening for the best nitrogen source revealed that peptone gave the highest activity of 28.5Uml-1, others are tryptone (25.5Uml-1), yeast extract (20.0Uml-1) and meat extract (22.4Uml-1), while NH4Cl showed the least activity of 7.0Uml-1. Also, 2.5% concentration of peptone gave the highest activity compared with other concentrations (0.5-4.5%) tested. Among the natural oils, olive oil gave the highest lipase activity of 25.2Uml-1 compared with other oils and 0.8% concentration of olive oil was found to increase lipase production by 36.2Uml-1. Other lipid sources observed were palm oil (20.5Uml-1), groundnut oil (19.5Uml-1), soy oil (16.4Uml-1) and crude oil (14.8Uml-1). The most suitable source of metal ion was MgSO4 (29.5Uml-1) followed by MnSO4 (27.9Uml-1), KCl had the least activity of 13.9Uml-1. Organic solvents on lipase activity revealed that methanol yielded higher lipase activity of 42.7Uml-1 than ethanol (30.5Uml-1), isopropanol (15.6Uml-1) and acetone (5.2Uml-1). The results promises high production of lipase with an inexpensive and simple medium containing palm oil, peptone, and magnesium sulfate, at pH 6.0 and 150 rpm for 48hrs (2days). This could be employed in the enzyme industry for commercial purpose.
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