Banana is one of the important fruit crop in tropical and subtropical countries suffering from numerous diseases. The panama wilt caused by Fusarium oxysporum f.sp cubense is the most destructive disease of banana. The pathogen has different races with different crop yield losses. Therefore we have studied on different isolates of the pathogen about the morphology and growth behaviour in different media for further studies on in vitro as well as in vivo management of this disease. After extensive survey in different districts, a total of 14 isolated of the pathogen were collected from different varieties. These different isolates were allowed to grow in different media. Among them potatoes dextrose agar was recorded as the best artificial media for growth and sporulation of all the isolates. Great variation was recorded both in growth (biomass production) and sporulation of the isolates. Production of conidia and ratio of macro and micro conidia are also significantly differed among the 14 isolates of Foc. So from these studies it may be reached to the point that there was probable variability in aggressiveness of different isolates of the pathogen as well as in the development of disease symptom on different varieties in different locations.

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