A set of 33 maize inbred lines were analyzed using 40 Simple Sequence Repeat (SSR) markers. Out of 40 SSR markers, 25 markers were found reproducible in which 18 were found polymorphic and 7 monomorphic. Total 40 alleles ranges from 2 to 3 with mean of 2.22 alleles per loucs were identified among 33 lines using18 polymorphic SSR markers. The average polymorphism Information Content (PIC) was 0.36 with range of 0.1 to 0.56. Unweighted Paired Group Method using Arithmetic Averages (UPGMA) cluster analysis grouped the genotypes into two major clusters. Cluster I was the biggest one with 25 genotypes which was further sub-divided into two sub-clusters with 21 and 4 genotypes, respectively. The major cluster II contained 9 genotypes in two sub clusters each with 7 and 2 genotypes. The identified clusters may find useful to attempt crossing programme in maize breeding programme. In this study, we found SSR as a good complementing tool along with morphological markers for maize genetic characterization.

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