In the present investigation, various factors influencing in vitro response were studied to develop an efficient reproducible regeneration protocol. Culture conditions for various stages of adventitious shoot regeneration including the induction, elongation and rooting of the elongated shoots were optimized. All the three genotypes exhibited varied response to explant type, kind and concentration of growth regulators and also recorded high degree of interaction effect. Apart from the shoot number, regeneration of quality shoots was considered by using combination of cytokinins. Good success was achieved for root regeneration and establishment of seedlings. Thus, the present study open ups the new horizons for genetic manipulation of recalcitrant pulse crops like chickpea through biotechnological interventions.

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