International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 6, Issue:11, November, 2017

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(11): 5328-5346

Evaluation of Rice (Oryza sativa L.) Germplasm for the Identification of High Folate Accession Using HPLC
S. Aiyswaraya1*, R. Saraswathi1, S. Ramchander1, N. Jaivel2, D. Uma3, D. Sudhakar4 and S. Robin1
1Department of Rice, Centre for Plant Breeding and Genetics, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu-641003, India
2Department of Agricultural Microbiology, Tamil Nadu Agricultural University,Coimbatore, Tamil Nadu-641003, India
3Department of Plant Biochemistry, 4Department of Biotechnology, Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University,Coimbatore, Tamil Nadu -641003, India
*Corresponding author

Folate is an essential micronutrient in human diet and its deficiency causes neural tube defects, coronary heart disease and certain forms of cancer, impaired cognitive functions. Humans depend on plant based sources for this nutrient. Rice is a major staple food but unfortunately with low folate content when compared to other plant based foods. Hence, screening and evaluation of available rice germplasm accessions for folate content is the basic step to identify folate rich accessions for further studies. In the present study, folate was extracted by a modified tri-enzyme treatment and assayed using HPLC C18 columns with a UV detector at 280 nm. The mean folate content of unpolished brown rice in 150 accessions were investigated and the results ranged from 9.721 µg/100 g to 29.284 µg/100 g revealing a three-fold difference between the lowest and highest values. Twelve lines had recorded significantly higher folate content (>20 µg/100 g), 136 lines had moderate (11 to 20 µg/100 g) and two lines had registered low level of folate (<10 µg/100 g). Cluster analysis revealed that, 150 accessions were grouped into three clusters at the similarity coefficient of 1.7. Cluster I consisted of five accessions viz., RG1 (Mapillai Samba), RG2 (CK275), RG3 (Senkar), RG4 (Murugankar) and RG162 (IR 64) with high folate range of 27.263 to 29.284 µg/100 g of sample. Cluster II consisted of 12 accessions in total with two sub clusters. Cluster III also comprised of two sub clusters in which, sub cluster I had 123 accessions with moderate level of folate content (12.516 - 18.603 µg/100 g) and the remaining 10 genotypes in sub cluster II had genotypes with lower values. These results suggested that accessions from high and low folate pool can be utilized in recombination breeding to enhance the level of folate and also to map the genomic regions associated with high folate content.

Keywords: Rice, Folate, Germplasm, High Performance Liquid Chromatography (HPLC).

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How to cite this article:

Aiyswaraya, S., R. Saraswathi, S. Ramchander, N. Jaivel, D. Uma, D. Sudhakar and Robin, S. 2017. Evaluation of Rice (Oryza sativa L.) Germplasm for the Identification of High Folate Accession Using HPLC.Int.J.Curr.Microbiol.App.Sci. 6(11): 5328-5346. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.