National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Phytases are enzymes that can hydrolyze phytic acid to less phosphorylated myo-inositol derivatives, releasing inorganic phosphate. In this study, phytase producing bacterium Pseudomonas aeruginosa and fungus Aspergillus niger isolated from poultry faeces were investigated and the production of phytase was optimized. Standard method was used to assay for Phytase activity. The time course production of phytase by P. aeruginosa showed that optimum phytase production was at 24 hours of incubation while that of A. niger was at 48 hours of incubation. Effects of agricultural substrates on phytase production by P. aeruginosa revealed the maximum activity (0.604µmol/min) of phytase with sorghum grain as agricultural substrate while maximum phytase activity (1.2µmol/min) by A. niger was obtained when millet grain was used. Effects of pH and temperature showed that optimum phytase activity from P. aeruginosa and A. niger was at pH 6 and 7 respectively while both preferred temperature of 37 ºC. Lactose was the best source of carbon for both. Yeast extract was observed as the best nitrogen for A. niger while urea for P. aeruginosa. In conclusion, The enzyme (phytase) was both thermostable and acid stable, can find applications in animal feed industry for improving nutritional status of the feed and combating environmental phosphorus pollution.
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