National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Arginine deaminase (ADI) is an important anticancer drug worldwide used in the chemotherapy of arginine-auxotrophic tumors, such as hepatocellular carcinomas and melanomas. To date, ADI obtained from Mycoplasma has been commercially used in clinics. However, low yield, high toxicity, short proteolytic and low serum tolerances are the major limitations of clinically available ADI. In the present investigation, we have described optimization of environmental and nutritional requirements for maximum biosynthesis of ADI from bacterium Pseudomonas aeruginosa PS2. We observed that batch time 25 h, inoculum age 20 h, 8% (v/v) inoculum size, pH 6.5 and temperature 37 °C were found as the most suitable operating conditions for ADI production. Galactose, peptone, KH2PO4 and L-arginine were found as the best carbon, nitrogen, mineral ion and inducer for ADI production, respectively. These results suggested that P. aeruginosa PS2 could be used for large-scale production of ADI but further studies are still required for strengthening the current findings which are underway in our lab.
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