National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Tomato (Solanum lycopersicum L.) is a major contributor to the fruit vegetable diet of humans. It is cultivated in essentially all countries either in fields or in protected culture. Tomato crop is affected by various pest and diseases. Among the diseases, Helicoverpa fruit borer and Fusarium vascular wilt is regarded as the most devastating to tomato around the world. The biological control method has been considered as a promising approach for the management of pest and diseases by enhancing the inherent systemic resistance. However the most of the approaches mainly biological control of pest and disease have used a single organism. This accounts for inconsistency in the antagonistic activity of biocontrol agent. So the combinatorial application of biocontrol agents could be viable for the management major pests and diseases in crop plants. In our study, both Pseudomonas fluorescens and Beauvaria bassiana possess excellent antagonistic activity against F. oxysporum f.sp. lycopersici and H. armigera respectively under in vitro. Combinatorial application of P. fluorescens and B. bassiana was found to reduce the pest and pathogen in tomato crop under glass house condition. Also, which imparts innate immune response by the production of defence related enzymes viz., peroxidase, polyphenol oxidase, superoxide dismutase and catalase on the treated host against necrotrophic pathogen and herbivore. The native polyacrylamide gel electrophoresis (PAGE) experiment showed that one to four isoforms of the defense related enzymes each with greater intensity were expressed in the given treatments. This is indicating that the restriction of insect pest and pathogens in tomato plants was mainly due to application of microbial bio-consortia.
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