National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
A survey was conducted in Tamil Nadu and Maharashtra to isolate the actinomycetes from the soil samples for study. Twenty seven cultures of actinomycetes were isolated, purified and designated as actinomycetes (A1 to A27). The test cultures A1 to A27 were taken for studying their acaridical activity. Preliminary screening revealed that five of the isolates were active against spider mite, Tetranychus urticae on okra. Out of the sixteen isolates A7 and A27 isolate showed highest activity against red spider mite, Tetranychus urticae. The elite isolate A7 and A27 was selected for further study. From the isolates only A7 showed maximum antimicrobial activity. This A7 isolate was selected and used for the present study to control red spider mite. Based on the mycelial and cellular morphology of A7 isolate observed under phase contrast microscope (100X), the actinomycete isolate was identified as Streptomyces fradiae. The 16s r DNA gene sequence A7 was found to be 98 per cent similar to S. fradiae. The 16s r DNA gene sequence A27 was found to be 98 per cent similar to S. fradiae. The phylogenetic tree obtained by applying the neighbour-joining method.
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