National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Nutrient paucity in the soil poses confront to global production of food. The use of synthetic nitrogen fertilizers to boost crop yield is a recurrent farming practice, despite of its unfavorable effects and hazard to the environment and human population. Rhizobium is gram negative bacterium which associates symbiotically with the roots of leguminous plants. Screening and selecting the rhizobial strain is important for biological nitrogen fixation. The present study was aimed to isolate and identify Rhizobium from Cicer arietinum (chickpea) root nodules by using CRYEMA medium. The result indicated that a bacterium from root nodules of chickpea does not absorb red color on YEMA medium and the milky white colony with spherical convex surface was isolated. Many biochemical tests of the isolated strain like oxidase, catalase and bromothymol blue were positive, while starch hydrolysis, lipase test, lysine decarboxylase and caseinase were negative which revealed that the strain isolated from chickpea plant belongs to Rhizobia species. The study indicated that all the strains grew well at pH 6 and 7, temperature 28º C to 30º C and at salt concentration of 1%. The strains showed resistance to the antibiotics, metal salts and salinity.
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