National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
In present study efforts were made to improve the regeneration protocol in the popular local soybean cultivars MAUS-71 and MAUS-162. Embryonic axis and Cotyladory node explants were taken and inoculated on different concentration of 2,4-D. Among those MS6D media containing 6mg/L 2,4-D were give best response for callus initiation. After proper callus formation these explants were transferred to shoot initiation and multiplication medium containing different concentration of BAP. MS media fortified with 3 mg/L BAP (MS3B) shows maximum shoot initiation and multiplication results. The Cotyladory node explants of cv. MAUS-71 shows maximum growth and development in all three types of media for all types growth conditions. The MS media fortified with 0.3 mg/L IBA as growth regulators gives better results for root formation in both types of explants and in both cultivars.
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