National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
This paper was carried out for detection of Rutin, Kaepferol, and Quercetin in corn silk, and an investigation of their effects (within crude) on the inhibition of urease enzyme and Klebsiella species urease. An HPLC has been used to detect the three flavonoid components in three extracts of corn silk (using 99.9% ethanol, 80% ethanol and water). The results were (0.012mg/L) of Quercetin in 99.9% ethanolic extract (99.9% EE). (0.1398 mg/L), (0.15 mg/L), and (0.11 mg/L) of Rutin, Kaepferol, and Quercetin respectively in 80% ethanolic extract (80% EE). (0.071 mg/L) and (0.091 mg/L) of Rutin and Kaepferol respectively in aqueous extract (AE). It is noticed that only 80% ethanol has an ability to extract such three flavonoids. All such extracts revealed an effective inhibition of urease enzyme in the Klebsiella species with (IC50 = 77.06, 13.54 +- and 35.93 mg/L) for 99.9% ethanol, 80% ethanol and aqueous extracts respectively, while standard urease inhibitor exhibits (IC50 = 453.4 mg/L) for thiourea (TU) as an example. Lastly, such extracts were utilized in the inhibition of pure enzyme and they exhibited an efficient inhibition of (IC50 = 402.8, 95.8, and 348 mg/L) for 99.9% ethanol, 80% ethanol and aqueous extracts respectively as compared with standard urease inhibitor of which gives (IC50 = 54077 mg/L) in thiourea (UT).
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