National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Multidrug resistant bacteria raise a serious clinical problem for treating infectious diseases worldwide. Different seaweed algae including Ulva lactuca (Chlorophyta), Petalonia fascia (Phaeophyta) and Gelidium spinosum (Rhodophyta) were investigated as natural sources for antibacterial compounds. Different solvents ethanol, methanol, acetone and water were used to extract the antibacterial substances from these seaweeds and were examined against urinary tract multidrug resistant bacterial isolates: Escherichia coli, Klebsiella pneumoniae, and Proteus mirabilis. The ethanolic extract of Ulva lactuca exhibited the most significant antibacterial activity against the tested urinary tract infection (UTI) bacterial isolates. The extract was purified and chemically analyzed using UV, IR, 1H NMR and GC-MS. The results indicated that the extract was aromatic ester derivative named Di-isooctylphthalate of molecular weight 390.56. PCR analysis of three virulence factors (adhesion, urease and hemolysin) were employed in order to genetically compare the virulence activities of the UTI isolates. The results showed that only faint PCR amplicon of hemolysin virulence gene was observed in P. mirabilis sensitive isolate, while two fragments were found in the multidrug and algal resistant corresponding isolates with ampilicon sizes of 375 and 875 bp.
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