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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 6, Issue:11, November, 2017

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(11): 1881-1888
DOI: https://doi.org/10.20546/ijcmas.2017.611.224


A Comparison of Antibody Titers in Brucellosis Affected Vaccinated Cattle, Unvaccinated Infected Cattle and Healthy Vaccinated Cattle by Agglutination Assays
Sugandha Raj1, Hari Mohan Saxena1* and S.T. Singh2
1Department of Veterinary Microbiology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India
2Department of Animal Genetics and Breeding, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India
*Corresponding author
Abstract:

The titers of anti-Brucella antibodies in naturally Brucellosis affected unvaccinated cattle, previously vaccinated infected cattle and normal healthy vaccinated cattle were estimated by common agglutination assays MAT, STAT, IHA. The mean antibody titers (log10) were found to be 2.856+0.47 by MAT, 2.041+0.39 by STAT and 2.471+0.51 by IHA, respectively in case of Brucellosis affected cattle which had been vaccinated during calf hood. The mean titers in case of naturally infected cattle which had never been vaccinated were 3.759+ 0.35 by MAT, 3.408+0.32 by STAT and 3.508+ 0.32 by IHA, respectively. The mean titers in healthy unaffected cattle vaccinated during calf hood were as follows 1.401+ 0.31 by MAT, 1.401+0.15 by STAT, and 1.45+0.16 by IHA, respectively. It was interesting to find that the antibody titers in naturally affected cattle which had never been vaccinated previously were very significantly (p<0.01) higher than those of Brucellosis affected cattle which had been vaccinated during calfhood and normal healthy vaccinated cattle unaffected by the disease. The mean titer in Brucellosis affected cattle which had been vaccinated during calfhood was very significantly (p<0.01) higher than that of normal healthy vaccinated cattle unaffected by the disease.


Keywords: Antibody titer, Brucellosis, Indirect Hemagglutination Assay, Microagglutination test, Standard Tube Agglutination Test.

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How to cite this article:

Sugandha Raj, Hari Mohan Saxena and Singh, S.T. 2017. A Comparison of Antibody Titers in Brucellosis Affected Vaccinated Cattle, Unvaccinated Infected Cattle and Healthy Vaccinated Cattle by Agglutination Assays.Int.J.Curr.Microbiol.App.Sci. 6(11): 1881-1888. doi: https://doi.org/10.20546/ijcmas.2017.611.224
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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