National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The research was conducted in commercial tissue culture laboratory, IGKV, Raipur, C.G. from the period of March 2015 to August 2017. Present investigation was conducted to assess the suitable explant for callusing using different treatment of auxin and cytokinin (NAA and BAP) in semi-solid Murashige and Skoog (MS) media. The results revealed that the meristem shoot found to be best for callusing. Among five different treatments MS medium with 2 mg/l BAP + 2mg/l NAA was found best for multiple shoot initiation for meristem shoot. For root induction MS medium with 1.0 mg/1 IBA + 500 mg/1 activated charcoal was found best with 96% per cent rooting and 5.42 mean number of roots. Further the regenerated plants were transferred to sterilized cocopit in poly house. After 1 week, plants were transferred to sterilized earthen pots containing a mixture of sand, FYM, soil (1:2:1) for acclimatization and transfer to the open field and 85 per cent plants were survived.
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