National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
An experiment on callus induction and indirect regeneration of strawberry cv. Chandler was conducted by taking different explants (internodal cuttings, matured leaves, young leaves, flower buds) were cultured on MS medium supplemented with different growth regulators and incubated in culture room for callus induction. Expansion and swelling of explants at the cut surface were noted and soft crystal, greenish compact calli were emerged from the cut surface within 3-4 weeks culture on MS medium supplemented with 2, 4- D (1.0 mg/l) along with BAP (0.1 mg/l). Also same medium resulted the highest callus induction (170.6 mg) along with 90 % callus induction frequency. Healthy calli were taken in a sterile petridish and chopped into small pieces with a fresh weight of approximately 100 - 120 mg, then inoculated on shoot regeneration medium containing different concentration of BAP (0.5, 1.0, 1.5, 2.0, 2.5 and 3.0 mg/l) and NAA (0.2 and 0.5 mg/l). Green shoot primordia developed on the surface of calli within 4-5 weeks. The number of shoots formed per callus was investigated after 30 days of culturing. BAP @2.5mg/l and NAA @0.5mg/l with the basal MS media showed the maximum response to regeneration via calli inducing an average of 5.1 shoot buds per culture within 4-5 weeks. Regenerated shoots were then transferred to MS medium with BAP and NAA for shoot multiplication and subsequent induction of best roots. The maximum percentage of rooting was observed in half strength MS media supplemented with 0.25 mg/l IBA and 2% sucrose. The developed plantlets through in vitro culture technique were acclimated and successfully transferred to in vivo condition for further evaluations. Analysis was carried out in completely randomized design with three replications for each treatment.
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