International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2022 - IJCMAS--ICV 2022: 95.28 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020] For more details click here

Login as a Reviewer

See Guidelines to Authors
Current Issues
Download Publication Certificate

Original Research Articles                      Volume : 5, Issue:5, May, 2016

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(5): 914-919

Serological Follow Up for Confirmation of Current Leptospirosis
J.Padma Kumari*, K. Usha Krishnan and T. Sheila Doris
Government Medical College, Omandurar Government Estate, Chennai, India Madras Medical College, Chennai, India
*Corresponding author

Leptospirosis is becoming an increasingly significant public health problem, particularly in tropical developing countries. The clinical diagnosis of leptospirosis is difficult due to the varied presentation of the symptoms of the disease and thus laboratory methods are needed to confirm it. Serology becomes an important diagnostic tool from the moment antibodies appear in detectable quantities in blood. Microscopic Agglutination Test(MAT) is the gold standard serological test but a single positive titre is, not diagnostic due to asymptomatic, past infection and cross reacting antibodies to other illness and should ideally be performed on paired serum samples and a demonstration of four-fold rise in titre of antibodies is essential to confirm current illness.  The aim of the present study is to  confirm the current leptospiral infection by demonstrating four- fold rise in titre of antibodies using paired sera by Microscopic Agglutination Test. The study population were 1209 patients from Chennai, with fever of one week duration with signs and symptoms of Leptospirosis and were screened for Leptospirosis by Macroscopic Slide Agglutination Test(MSAT). Out of 1209 patients, 237 patients who were positive by screening test MSAT were followed-up to obtain paired serum sample. Second sample was collected from 158 patients. The acute phase serum sample and the paired sera were analysed by serological test MAT. Out of 158 cases followed up, 142(89%) cases showed a four fold rise in titre of antibodies and were confirmed as having current leptospiral infection. If only a single sample was used to diagnose leptospirosis  16 cases would have been  false positive for leptospirosis and the true cause of the febrile illness would have been missed.  So it is mandatory that we examine two samples one in acute phase and one during convalescent phase thus confirming the current leptospiral infection.

Keywords: Leptospirosis, MSAT,MAT,paired sera,follow up.

Download this article as Download

How to cite this article:

Padma Kumari, J., K. Usha Krishnan and Sheila Doris, T. 2016. Serological Follow Up for Confirmation of Current Leptospirosis.Int.J.Curr.Microbiol.App.Sci. 5(5): 914-919. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.