National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Green synthesis of nanoparticles is one of the most effective processes at a very affordable cost. Green synthesized nanoparticles have wide range of antimicrobial property. Thevetia peruviana is commonly called as yellow oleander has chemicals (thevetin A and thevetin B) which help in bio-reduction of metals and antimicrobial property. Ripen yellow oleander seeds were used to prepare extract. Silver nanoparticles were synthesized by exposing AgNO3 (1mM) with T. peruviana seeds extract (10 %) to the bright sunlight, autoclaved method and combination of both methods. Colour change was noted from light milky white to dark orange in all the three methods tried. Synthesized silver nanoparticles were characterized by UV-Visible spectrophotometer and Atomic Force Microscope. Absorption spectra of sunlight exposed AgNPs was 425 nm, autoclaved method 415-420 nm and combination of both recorded 350 nm. Size of AgNPs varied from 50 to 200 nm and spherical to irregular in shape. Antifungal efficacy of AgNPs after 24 h of incubation showed more than 95.00 per cent inhibition of Curvularia lunata (Leaf spot of maize) in sunlight and autoclaved method while combination of both methods recorded 58.68 per cent and control (no inhibition). Inhibition of spore germination was on par with fungicide (propiconazole @ 0.1 %). Silver nanoparticles synthesized from sunlight and autoclaved method were more effectively inhibited spore germination compared to combination of both methods and control.
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