National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
To identify laccase producing actinomycete strains a different approach was used in this study. Actinomycete strains were first grown in the presence of an inducer to better the enzyme induction. Later these strains were screened for the production of laccase enzyme which is very scarce in this group of bacteria. 62 actinomycete strains were isolated from the soil and mangrove samples collected from various locales. Aromatic inducers like guaiacol, 2,5 xylidine and tween 80 at the concentrations of 1mM were incorporated in the liquid medium. Also a lignin rich natural inducer pearl millet (bajra) powder at the concentration of 8% was incorporated in the liquid medium. The induction process with the variety of inducers was carried out for all the isolates for 2 weeks. After induction, enzyme activity was detected qualitatively by incorporating guaiacol in the medium. One isolate showed a reddish brown halo around the colony after 72 hours of incubation at 370C indicating laccase positive activity. Laccase activity of this isolate was further confirmed by decolorization of two dyes RBBR and congo red.
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