National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Native plant growth promoting rhizobacteria are unable to compete with other bacterial strains and hence cannot express their full potential in realising the beneficial effects. Hence, inoculation of plants with target microorganism is necessary to exploit full potential for yield enhancement. This study was aimed at standardizing the technique for isolation of Rhizobium species from root nodules of lentil so as to explore and maximize their contributions to soil nitrogen fertilization in place of synthetic fertilizers. A total of five nodulated healthy plants collected from North-eastern part of Uttar Pradesh, India were sterilized and macerated under aseptic condition followed by streaking one loop full suspension in a zig-zag manner using the inoculation loop on YEMA medium in laminar air flow and incubated in a B.O.D. incubator at 28±2ºC for 24 hours. Growth on YEMA plate was observed after the said incubation period and then maintained at 4ºC in 20% (w/v) glycerol at -80ºC for long-term storage. Vigorous growth of bacteria was seen in next 24 to 48 hours. Bacterial colonies were found to have distinct colour, morphology, appearance and texture on agar plates.
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