Enzymes in the gut of three different stages (juvenile, pre-clitellate, clitellate) of the earthworm species Perionyx ceylanensis, reared in the vermibed substrates namely, teak leaf litter (TLL), paper mill sludge (PMS) and pressmud (PM) in combination with cowdung were analysed. The enzymes, amylase (EC 3.2.1.1), cellulase (EC 3.2.1.4.), protease (EC 3.4.0), phosphatase (EC 3.1.3.1, 2) and invertase (EC 3.2.1.20) were analysed from gut contents of the earthworm reared in TLL, PMS and PM on 60^th day. The activity of the enzymes amylase, cellulase, protease, phosphatase and invertase were invariably observed to be more in clitellate adults followed by pre-clitellates and juveniles. The results also showed variation in enzyme activities with respect to different vermibed substrates.

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