National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The efficacy of different microbial strains for potassium dissolution from insoluble soil mineral (mica) were evaluated in vitro (both plate and broth media assays). Nine microbial strains and 0.1% of chemical source (mica) in three replications were used. Colony and halo diameters were measured after incubating the plates for 72h in incubator. Further, potassium solubilizing ability of microbial strains was studied with mica solution in broth assay. Solubilization potential was assessed both qualitatively and quantitatively under in vitro conditions. The laboratory stock cultures (KSB-W1 (Bacillus sp), KSB-PD-3-A, (Bacillus sp), KSB-NP-3 (Bacillus sp), KSB-PD-1-A (Pseudomonas sp), KSB-M-1 (Pseudomonas sp), KSB-M-2 (Pseudomonas sp), KSB-PD (Sinorhizobium metallidans), KSB-PD-1-B (Sinorhizobium metallidans), KSB-M-3 (Sinorhizobium metallidans)) were obtained from All India Network Project on Soil Biodiversity-Biofertilizers, Parbhani and SKUAST, Kashmir and were selected on the basis of their potash solubilizing ability in laboratory condition. Results indicated that, by plate assay among the microbial strains Pseudomonas sp (KSB-PD-1-A) formed the highest colony diameter (1.17 cm) and halozone diameter (3 cm) with mica amended media. Pseudomonas sp (KSB-PD-1-A) formed significantly superior and greater solubilization index (3.57) and solubilization efficiency (257.32%) as compared to other microbial strains. In Broth culture assay, Maximum potassium solubilization was observed with the Pseudomonas sp (KSB-PD-1-A) in mica amended media compared to control. Maximum reduction in pH was also recorded in Pseudomonas sp (KSB-PD-1-A) with mica amended media compared to control.
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