National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Development of QPM (Quality Protein Maize) with high lysine and tryptophan is foremost important task in enhancing nutritional quality in maize through breeding programme. Marker assisted selection is the most feasible way of developing QPM hybrids in short time. The present investigation deals with conversion of elite normal maize inbred lines BML6 and BML7 (parental lines of DHM117 hybrid) into QPM lines using marker assisted selection. The nutritional quality of maize is enhanced by introgression of the opaque2 (o2) gene along with numerous modifiers for kernel hardness. To improve the efficiency of QPM breeding, the utility of three simple sequence repeat (SSR) markers viz. umc1066, phi057 and phi112 were used in selection and introgression of the opaqaue2 gene. Polymorphism was detected between recipient parents (BML7 and BML6) and donor parent (CML181) with umc1066 SSR marker. Foreground selection was exercised in each generation using opaque2 specific marker umc1066 while background selection was carried out in BC1F1 and BC2F1 generations to recover the recurrent parent (RPG) genome using SSR markers distributed across the genome. In BC2F1 the recovery of recurrent parent was between 90 to 93% and the plants with highest recovery were selfed to generate advanced generations (BC2F2 and BC2F3). Kernels were screened for endosperm hardness using light box and kernels showing less than 25% opacity were selected. Rigorous phenotyping was done for plant characters and tryptophan was estimated using colorimetric method. Tryptophan content varied from 0.76% to 0.95% in BC2F3 derived population of BML6 and 0.72% to 0.92% in BC2F3 derived population of BML7. Normal looking converted inbreds (CBML6 and CBML7) with high tryptophan and high yield were used for reconstitution of the QPM version of DHM117 maize hybrid. Two cross combinations CB6-36 × CB7-28 and CB6-36 × CB7-59 exhibited relatively higher tryptophan content and on par grain yield per plant compared to check DHM 117 which need to be further tested under multi-location trials prior to commercial exploitation.
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