National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
In this study, we aimed to increase the protease production of some Bacillus isolates obtained from Jeddah City. Among fifty-four isolates, four isolates were the highest productive, J1, KA6, F5 and M4. They gave 10.76, 10.50, 10.30 and 9.36 U/ml, respectively. They were assigned as Bacillus BS, BP, BT and BL. The highest productive strains were subjected to mutagenesis using UV irradiation and Ethidium bromide. Out of fifty-two mutants, four mutants exhibited high efficiency of protease production. They were BL4-1, RS6-1, BETL0-1 and BETP40-2.They gave 25.98, 22.10, 19.74 and 14.92 U/ml, respectively. BL4-1 gave productivity threefold than Bacillus BL parental strain while RS6-1 gave two fold than Bacillus BS. Generally, UV irradiation was more efficient than EtBr in obtaining mutants with higher efficiency. Top highest mutants, BL4-1, RS6-1, and their parental strains were subjected to plasmid curing. Curing of Bacillus BL by elevated temperature has no effect on production while curing with acridine orange; production was increased. BL4-1 mutant showed great decrease in productivity by acridine orange compared to elevated temperature. In case of Bacillus BS parental strain, productivity was increased after curing with acridine orange while with elevated temperature the increase was medium. Production was not significantly affected by acridine orange in RS6-1 mutant while decrease sharply by elevated temperature.
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