International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 5, Issue:5, May, 2016

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(5): 619-635

Enhancement of Protease Production of Some Bacillus spp. Isolated from Various Regions in Jeddah City
Effat A.M. Soliman1,2*, O.I.M. El-Hamshary1,2  and Reem S.M. Batayyib2
1Microbial Genetic Dept., National Research Centre, 33 El-Bohouth St
(Former El-Tahrir St.) Dokki, Giza, Egypt, P.O. 12662
2Biological Science Dept., Fac. of Science, King Abdulaziz University, Jeddah, KSA
*Corresponding author

In this study, we aimed to increase the protease production of some Bacillus isolates obtained from Jeddah City. Among fifty-four isolates, four isolates were the highest productive, J1, KA6, F5 and M4. They gave 10.76, 10.50, 10.30 and 9.36 U/ml, respectively. They were assigned as Bacillus BS, BP, BT and BL. The highest productive strains were subjected to mutagenesis using UV irradiation and Ethidium bromide. Out of fifty-two mutants, four mutants exhibited high efficiency of protease production. They were BL4-1, RS6-1, BETL0-1 and BETP40-2.They gave 25.98, 22.10, 19.74 and 14.92 U/ml, respectively. BL4-1 gave productivity threefold than Bacillus BL parental strain while RS6-1 gave two fold than Bacillus BS. Generally, UV irradiation was more efficient than EtBr in obtaining mutants with higher efficiency. Top highest mutants, BL4-1, RS6-1, and their parental strains were subjected to plasmid curing. Curing of Bacillus BL by elevated temperature has no effect on production while curing with acridine orange; production was increased. BL4-1 mutant showed great decrease in productivity by acridine orange compared to elevated temperature. In case of Bacillus BS parental strain, productivity was increased after curing with acridine orange while with elevated temperature the increase was medium. Production was not significantly affected by acridine orange in RS6-1 mutant while decrease sharply by elevated temperature.

Keywords: Bacillus spp.,UV irradiation, Ethidium Bromide, Plasmid curing,  Protease.

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How to cite this article:

Effat A.M. Soliman, O.I.M. El-Hamshary  and Reem S.M. Batayyib. 2016. Enhancement of Protease Production of Some Bacillus spp. Isolated from Various Regions in Jeddah City.Int.J.Curr.Microbiol.App.Sci. 5(5): 619-635. doi:
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