National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
This study aims to determine antimicrobial activity of the leaf extract – fractions of Vernonia calvoana against selected stock cultures in Microbiology Laboratory, Cross River University of Technology, Calabar. Agar disk diffusion method was used for screening of extract – fractions against Escherichia coli, Staphyloccus aureus, Salmonella typhi, Candida albicans, Aspergillus flavus, Pseudomonas aeruginosa and Helicobacter pylori. Out of the seven, three of the microorganisms were used to determine the minimal inhibitory concentration (MIC) owing to their profound inhibition. The crude extract, 30% and 100% methanol fractions were assayed for antimicrobial activities. The crude extract exhibited the highest antimicrobial potency against S. aureus, E. coli, and P. aeruginosa with inhibition zone diameter ranged from 20 - 22mm. The inhibitory effect of 100% methanol fraction was recorded with inhibitory zone diameter ranged from 12 – 19mm; and the 30% methanol fraction slightly inhibited with zone diameter ranged from 10 – 15mm against the test microorganisms. Vernonia calvoana plant showed antimicrobial potency against the selected stock cultures and probably could be used to control infections associated with these organisms.
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