National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The present experiment was conducted to standardize the protocol for in vitro mutagenesis of gladiolus hybridus varieties ‘Punjab Glance’ and ‘Sylvia’. The nodal buds were irradiated with different doses of gamma rays (Co60) 0, 5, 8, 10 and 12 Gy. Irradiated explants were then cultured aseptically on MS basal medium supplemented with 5 mg/l (BAP) + 2mg/l (IBA) and agar-agar to induce shoot and root proliferation. Explants treated at higher doses (10 and 12 Gy) showed deleterious effects of ionizing radiation. Morphological abnormalities in terms of stunted growth were observed at 10 and 12 Gy of gamma rays. The minimum time taken to shoot and root (14.07), (15.06) formation, days to cormel formation (60.04 days) at 8 Gy. Shoot length (4.04 cm), number of shoots (25.07), root length (4.18 cm), number of roots per (10.04). In vitro-raised mutant cormels and non-irradiated (control) plants were transferred to plastic pots one month after acclimatisation under laboratory conditions and examined for their morphological traits. These mutated cormels were multiplied on a large-scale through micropropagation and evaluated for their stability. This study developed a mutagenesis protocol that could be used to develop novel colour mutants in gladiolus.
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