National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Intestinal contents from freshwater fish (Catla catla) (n=150) were bacteriological cultured for Escherichia coli and confirmed by PCR targeting E16S gene. A total of 104 E. coli isolates were recovered and analyzed for the presence of extended-spectrum beta-lactamases (ESBLs), phenotypically by disc diffusion method and genotypically by multiplex PCR targeting blaTEM, blaSHV, blaOXA, blaCTX-M and blaAmpC beta-lactamase (bla) genes. Phenotypic beta-lactam resistance was detected in a total of 16 isolates, of which nine isolates carried ESBL phenotype by combination disc method. Multiplex PCR assay revealed presence of blaAmpC, blaTEM, blaCTX-M, blaSHV and blaOXA genes in 13, 13, 9, 6 and 4 isolates, respectively. Serotyping of beta-lactam resistant E. coli isolates (n=16) revealed O120 (2 isolates), O141 (2), rough (2), O63 (1), O126 (1) and untypable (8) serotypes. This was the first report on the phenotypic detection and molecular confirmation of ESBL producing E. coli from freshwater fish in Andhra Pradesh.
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