International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 5, Issue:5, May, 2016

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(5): 127-137

Screening of Potent Laccase Producing Organisms Based on the Oxidation Pattern of Different Phenolic Substrates
Sheena Devasia* and A. Jayakumaran Nair
Department of Biotechnology, University of Kerala, Kariavattom, Trivandrum, Kerala, India
*Corresponding author

Soil samples were collected from different forest areas of Kerala and screened for laccase activity using guaiacol plate assay technique. An increased concentration of guaiacol was used for the isolation of resistant strains. Substrate oxidation studies were carried out and the potent organisms were taken for enzyme production studies. The organisms, isolated by the screening strategies, were found efficient laccase producers. The potent organisms were identified as Arthrographis sp. and Enterobacter cloacae. The enzyme production rate of Arthrographis was found to increase logarithmically and a maximum quantity of 53 U/ml was obtained and the bacterial strain, Enterobacter cloacae, gave a maximum laccase activity of 8U/ml. The difference in the pattern of substrate oxidation by laccase from different organisms is paid attention in the study.

Keywords: Arthrographis, bacterial laccase, Enterobacter cloacae,guaiacol, laccase, lignin, oxidoreductase.

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How to cite this article:

Sheena Devasia and A. Jayakumaran Nair. 2016. Screening of Potent Laccase Producing Organisms Based on the Oxidation Pattern of Different Phenolic Substrates.Int.J.Curr.Microbiol.App.Sci. 5(5): 127-137. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.