The objective of this study is by focusing on the use of biofilm and probiotics on prevention and treatment of mastitis in cows.  Fifty one isolates of Staphylococcus aureus were collected from different local areas in Iraq.  In this study, forty eight Staphylococcus aureus were producing biofilm in different thickness while the remaining three isolates couldn’t produce biofilm.  Six of Staphylococcus aureus isolates ( from the forty eight) had higher thickness (3mm) of biofilm in which the protein concentration were 62,66,70,72,80 and 85 mg /ml., subsequentially.  Staphylococcus aureus isolates that produce high thickness of biofilm appeared to be more resistant to most common antibiotics and had significant differences at 18 ±0.25 with the  level  p< 0.05.

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