National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Xylose reductase (XR) is responsible for biotransformation of xylose to xylitol and it has gained significant position amongst industrial enzymes due to its role in meeting the demand of xylitol by utilizing agriculture waste. Xylitol is a rare pentose sugar alcohol having a number of therapeutic and pharmaceutical applications. There is a paradigm shift for biotechnological production of xylitol over conventional chemical method. Amongst biotechnological methods, enzymatic method is efficient over whole cell method for industrial xylitol production due to increased product yield and easy recovery of purified product. But, an efficient cell disruption strategy is critically required for the efficient recovery of intracellular XR from the microbial cell. The focus of present study is to test various physical, chemical and enzymatic methods for the disruption of cells of novel isolated Pseudomonas putida BSX-46 for maximum release of xylose reductase. Amongst all the methods adopted, sonication treatment given to cells pretreated with EDTA and β-mercaptoethanol was found to be most effective for maximum release of XR with an activity of 48.70±0.05 IU/mg of cells. The findings from the present study can result in development of an efficient method for making use of XR for industrial production of xylitol.
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