National Academy of Agricultural Sciences (NAAS)
|
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Gaza strip is a narrow piece of land lying in the coast of the Mediterranean Sea. Its position on the cross roads from Africa to Asia made it a target for occupiers and conquerors over the centuries. The Gaza Strip is situated in the south part of historical Palestine and southeast of the mediterranean. Ten soil samples were collected from different agricultural and non-agricultural soils from different locations in Gaza strip, and all samples of Bacillus thuringiensis strains were isolated from soil. B. thuringiensis is a gram positive and spore forming bacterium. The most of the natural habitat for this bacterium is soil and it is capable of producing the diversified varieties of crystal proteins (cry proteins) with insecticide property. Polymerase Chain reaction (PCR) is a molecular tool widely used to characterize the insecticidal bacterium B. thuringiensis. This technique can be used to amplify specific DNA fragments and thus to determine the presence or absence of a target gene. The molecular identification of Bt cells through the PCR analysis of the delta-endotoxins genes coupled to ribotyping, is an innovative method, that has enabled the identification of this organism into wetland environments.
Citations |
 |
 |
 |
 |
