National Academy of Agricultural Sciences (NAAS)
|
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
In this study, the soil samples were collected from four different mountain sites of Hadhramout governorate in Yemen to investigate the diversity of actinomycetes. Soil samples were analyzed for physicochemical characteristics (temperature, pH and nature of the soil samples). Site soil color varied from brown to light brown and dark brown, temperature of the locations ranging from 33ºC to 39.1ºC and pH from 7 to 9. Actinomycetes were isolated by serial dilution and plating method on starch casein agar media. Total 56 species were isolated on the bases of colony characteristics on starch casein agar and all the isolates were screened for antibacterial activity by agar disk diffusion method (ADD) and agar well diffusion method (AWD). So, 30% isolates have shown activity against Bacillus subtilis by ADD and 14% isolates by AWD. For Staphylococcus aureus 24% isolates had activity by ADD and 8% isolates had activity by AWD. There were 33% isolates showing activity against Pseudomonas aeruginosa by ADD and 15% isolates by AWD. The activity was low against Escherichia coli, only 24% isolates have shown activity by ADD and 11% isolates have shown activity by AWD. In this study, we noticed MKr123/1 isolate has a strong activity against both Gram positive and Gram negative bacteria so that MKr123/1 isolate was selected and it is identified as Nocardia otitidiscaviarum.
Citations |
 |
 |
 |
 |
