National Academy of Agricultural Sciences (NAAS)
|
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
A novel Streptomyces sp. Ru87 was isolated from Egyptian soil and showed significant inhibition activities against a number of food and blood borne pathogens. Morphological and chemotaxonomic characteristics showed that Ru87 belongs to genus Streptomyces. 16 S rRNA gene sequence (KY775360), showed 98% identity with Streptomyces cacaoi strain NBRC 12748. However, phylogenetic analysis indicated that it is distinct from the known species. A cultured extract of this strain inhibits the growth of Staphylococcus aureus and some other foodborne and blood borne pathogens. The highest yield of antimicrobial agent was obtained after 6 days of culturing 4 x 105 CFU/ml in starch casein broth media with agitation (150 RPM) at 30oC, pH 7.0. The analytical studies using Thin Layer Chromatography (TLC) and High Performance Liquid Chromatography (HPLC) illustrated that the final extract represent one pure component. The antimicrobial activity of bioactive fraction was investigated using minimum inhibitory concentrations (MIC) and reporting 24 µg active of fraction/ml against S. aureus. Partial characterization was conducted using Mass spectroscopy, IR and 1H, 13C Nuclear Magnetic Resonance (NMR) indicated for the first time that Streptomyces sp. Ru87 produce antibiotics related to aromatic peptides.
Citations |
 |
 |
 |
 |
