National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
In the present experiment the depletion profile study of Albendazole and its metabolites (ABZSO and ABZSO2) were carried out in the plasma of Pangasianodon hypophthalmus. The experiment consists of three treatment groups and control. A 20mg/kg body weight dose of ABZ was given through intraperitoneal, intramuscular and orally respectively to the three treatment groups while no drug was given to control group. The blood samples were collected at 0.5, 1, 2, 4, 8, 12, 24, and 48h intervals to determine the ABZ, ABZSO and ABZSO2 using High Performance Liquid Chromatography (HPLC) having C-18 reversed–phase analytical column. The samples were processed by mobile phase of Methanol-Water (60:40) at a constant flow rate of 1 ml/ min and detected at 295 nm using a UV/Visible detector. The drug was detected in the plasma of orally given treatment group only. The Maximum concentration (Cmax) of ABZ 3.461± 0.06 μgml-1 found at 8 h (Tmax) and retention time (RT) of 23.88 minutes. For ABZSO Maximum concentration (Cmax) detected was 0.037 ±0.009 μgml-1 at 8 h (Tmax) with RT of 5.61minutes and ABZSO2 Cmax found was 0.023 ± 0.007 μgml-1 at 12 h (Tmax) with RT of 19.4 minutes. The concentration of metabolites reached to the levels of negligible after 48 h. The present study reveals that giving ABZ through orally is efficient method for treatment of helminth infections in fishes.
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