National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The study was planned to fulfill the objectives like to isolate the cellulolytic bacteria and fungi, determination of the cellulase production from the colocasia as a substrate using the isolated cellulolytic fungi and determination of the optimal condition for the cellulase production condition such as temperature (30ËšC, 35ËšC, 40ËšC, 45ËšC and 50ËšC), pH (Alkaline and Acidic), Time (24, 48, 72 hrs) and substrate (Colocasia Leaf, Root, Stem) and estimate CMCase activity, reducing sugar and protein. The cellulase biomass was also measured on dry weight base. This study revealed that colocasia leaf, stem and root which are very economic as well as produce large amount of cellulase enzyme when hydrolysed by cellulytic fungi in acidic pH. The suitable conditions for cellulase production are 50°C temperature and 24 hrs, 48 hrs and 72 hrs are suitable for stem, root and leaf, respectively. So instead of being left behind for natural degradation, this can be utilized effectively under these conditions, to produce cellulase.
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