National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Stevia rebaudiana is a herbaceous perennial plant of Asteraceae family and an excellent substitute of sugar. Increasing demand of quality panting material has emphasized the need of rapid and mass multiplication through plant tissue culture technology. Therefore, high frequency mass multiplication of Stevia was established using in vivo and in vitro nodal explants. MS basal medium supplemented with 2.0 mg/l TDZ induced maximum shoot induction (68.3%) from in vivo nodal segments and 2.5 mg/l TDZ showed 100% shoot induction from in vitro nodal explants. The highest shoot multiplication (7.33 shoots/explant) was found on MS medium supplemented with 1.0 mg/l BAP+ 0.5 mg/l Kin. Root induction was performed on MS basal medium supplemented with different concentration of IAA, IBA and NAA. Quality root formation with respect to root length and root number per microshoots was observed on hormone free MS basal medium. Rooted plantlets were hardened in vermiculite and cocopeat in the ratio of 1:1 for 3 weeks and successfully acclimatized in field condition. Assessment of clonal fidelity of in vitro raised plantlets was performed by RAPD and ISSR molecular analysis which revealed that all the regenerated plants of stevia through tissue culture were true-to-type compared to mother plants. The protocol developed in the present study could be used for production of approximately 2400 true-to-type plants in 12 week from single microshoot from 4 subcultures.
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