National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Hydrogen peroxide is well known to produce reactive oxygen species which cause damage to nucleic acids, proteins and disintegrate plasma membrane. It is also responsible for aging and neoplasmic progressions. The present study investigated the antigenotoxic effect of Withania somnifera extract against DNA damage induced by hydrogen peroxide in cultured human peripheral blood lymphocytes (PBL) using sister chromatid exchange (SCE) assay as a biomarker of genotoxicity. Heparinised fresh blood from healthy individuals was treated with hydrogen peroxide in presence of Withania somnifera extract in order to observe its antigenotoxic potential. A significant (p<0.05) dose dependent increase in frequency of SCEs was observed as the concentration of hydrogen peroxide increases. However, Withania somnifera (35 µg/ml and 70 µg/ml) in presence of 20 µM of hydrogen peroxide had significantly reduced (p<0.05) the frequencies of SCEs as compared to PBL exposed to hydrogen peroxide only. Hence, our findings suggest that Withania somnifera can protect against DNA damage induced by hydrogen peroxide in cultured human PBL.
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