National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Plants are well known as vectors for transmission of human enteric pathogens and exaggerate the outbreak events. Salmonella enterica, rod shaped, facultative anaerobic bacteria interferes with the microbial safety of raw vegetables. This study explored three sources: Village fields, Supermarkets and Street Vendors to ensure the microbial risk among seven raw vegetables (N=725). Water samples of the hand pumps sourced from village fields showed total coliforms >10ml-1 (75.6-98.5%) and faecal coliforms >10ml-1 (59.6-91.2%). The highest Aerobic Mesophilic count (6.66 log cfu g-1) in spinach samples and highest Salmonella enterica count (5.09 log cfu g-1) in tomato samples procured from vendors was observed. Resistance to Lyncosamides, Tetracycline, Imipenem, Metronidazole and Cloxacillin has been observed. Twenty isolates were found in high Multiple Antibiotic Resistance (MAR) index range 0.36-0.8. Polymerase Chain Reaction (PCR) analysis has revealed the presence of sipB (100%), sopB (100%), spiA (100%), invA (100%) except hilA gene which was found absent in all isolates. The in-vivo expression of these virulence genes and resistance to multiple antimicrobials can potentially threat the healthy being of consumers.
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