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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 6, Issue:8, August, 2017

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(8): 2971-2979
DOI: https://doi.org/10.20546/ijcmas.2017.608.356


Carpogenic Germination and Viability Studies of Sclerotia of Sclerotinia sclerotiorum Causing Lettuce Drop
Meenu Gupta* and Kirtipal Singh
Department of Vegetable Science, Dr YS Parmar University of Horticulture and Forestry, Nauni, Solan HP-173230- India
*Corresponding author
Abstract:

Lettuce is one of the most widely consumed vegetable throughout the world. Amongst various diseases infecting this crop, lettuce drop caused by Sclerotinia sclerotiorum is one of the most prominent and destructive one. Lettuce drop pathogen, S. sclerotiorum produces black, hard survival structures known as sclerotia. In the present studies, carpogenic germination of sclerotia by producing apothecia and releasing ascospores as well as viability of sclerotia was studied. Sclerotia after germination formed funnel shaped cup i.e. apothecium at the tip which were brown in color and were round or globose type. The length of apothecia varied from 4-19 mm, whereas diameter of the apothecial discs ranged from 3-8 mm with number ranging from 1-10 per sclerotia. Viability studies revealed that with the increase in duration of water soaking from 4 days to 32 days, there was a gradual decrease in germination of sclerotia of S. sclerotiorum. 20oC temperature was found to be optimum resulting in maximum sclerotial germination (99.73%) followed by 25, 15, 10 and 30oC temperature. Soil depth (0 to 12cm) was inversely proportional to sclerotial germination and sclerotia placed at soil surface (0 cm soil depth) resulted in highest sclerotial germination (93.75 %) followed by 2, 4 and 6 cm soil depths.


Keywords: Carpogenic germination, Sclerotinia sclerotiorum, Lactuca sativa, Lettuce drop, Viability studies.

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How to cite this article:

Meenu Gupta and Kirtipal Singh. 2017. Carpogenic Germination and Viability Studies of Sclerotia of Sclerotinia sclerotiorum Causing Lettuce Drop.Int.J.Curr.Microbiol.App.Sci. 6(8): 2971-2979. doi: https://doi.org/10.20546/ijcmas.2017.608.356
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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