National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
In the present study About 24 isolates of fungal bio-control agent particularly Trichoderma spp and 12 bacterial isolates were isolated from the rhizosphere of tomato which was identified based on morphological and cultural characteristics. Among 24 isolates of Trichoderma spp ten and twelve bacterial isolates were identified with antagonistic ability and tested against the test soil borne pathogens P. debaryanum, R. solani and S. rolfsii. The Trichoderma harzianum-7 and Trichoderma harzianum-1 were found effective in inhibition of mycelium (80.03), (62.53 and 95.1) against P. debaryanum, R. solani and S. rolfsii respectively under in vitro conditions. The efficient isolates further screened for functional plant growth promoting properties. Among twelve bacterial isolates, Pseudomonas fluorescence-3 recorded highest solubilization zone followed by Pseudomonas fluorescence-2. All the bio-agents showed positive results to ammonia production except Trichoderma harzianum-6. Similarly all the bio-gents exhibited positive results for HCN production. Highest HCN production was observed with the Trichoderma viride-2, 9, 10 and Trichoderma harzianum-4, 7, 8, 13 and bacterial isolates of Bacillus subtilis-2, 4 and Pseudomonas fluorescence-10. IAA production was observed with Trichoderma but the highest IAA production was noticed with the bacterial isolates of Bacillus subtilis-5, Pseudomonas fluorescence-2 and 12. All the bacterial isolates showed siderophore production in the plate assay of which the bacterial isolates of Bacillus subtilis-4, Pseudomonas fluorescence-1 showed prominent orange colour zone. The Trichoderma viride 9 and Trichoderma harzianum-2 showed lower production of siderophores.
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