National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The use of transferable cross-species/genera SSR markers is an alternative strategy to ensure availability of markers in genomic resources-limited crops such as dolichos bean. A total of 275 cross legume species/genera SSR markers were examined for their transferability to dolichos bean. 126 of 275 cross legume species/genera SSR markers (45.81%) were found transferable to dolichos bean. Transferability varied with cross legume species/genera with 83.33% of Medicago truncatula, 78.57% of greengram, 77.77% of chickpea, 53.33% of soybean and 33.33% of cowpea SSR markers being transferable to dolichos bean. The extent transferability of SSR markers based on simple di-/tri-nucleotide repeat motifs was higher than those based on penta-/tetra-/complex nucleotide repeat motifs. Of the 126 transferable SSR markers, 64 produced amplification in all the 14 advanced breeding lines with an average of 2.42 alleles per SSR locus. Six of the 64 SSR markers were most informative with four allelic variants.
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