Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
Home Editorial Board Current Issue Special Issue Archives Indexing Submit Article
Instruction to Authors Manuscript Charges Copyright Form Model Manuscript Process Flow (Time Frame) FAQ
Reviewer Guidelines Selection of Reviewers Join our Team of Reviewers Apply as Reviewer Reviewer Login
Editorial Review Policy Embargo Policy Ethical Expectations Consent For Identifying Information Informed Consent Manuscript Withdrawal Policy Peer Review Process Plagiarism Policy Ethical Violations Appeal Policy Against Editorial Decision Advertising Policies Authorship Policy
Access Policies Authorship Publication Policy Publication Ethics Complaints Policy Confidential Information Conflicts of Interest Policy Consent for Copyrighted Material Copyright Policy Corrections and Retractions Digital Preservation Policy Research On Human And Animal Studies Open Access Policy
Publication Ethics Contact
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2022 - IJCMAS--ICV 2022: 95.28 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020] For more details click here

Join as a Reviewer

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here
ICV 2022: 95.28
Index Copernicus ICI Journals Master List 2022 - IJCMAS--ICV 2022: 95.28
For more details click here
Announcement

See Guidelines to Authors
Current Issues
Download Publication Certificate

Original Research Articles                      Volume : 6, Issue:8, August, 2017
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38
Metrics and citations

Int.J.Curr.Microbiol.App.Sci.2017.6(8): 90-100
DOI: https://doi.org/10.20546/ijcmas.2017.608.012


Extraction and Purification of Lipopolysaccharide of Klebsiella pneumoniae Isolates
Abeer Ghassan Munther Al-Agha*
Department of Microbiology, College of Veterinary Medicine, University of Diyala, Diyala, Iraq
*Corresponding author
Abstract:

The genus Klebsiella contains a number of opportunistic pathogens which cause several serious infections, including pneumonia, bacteremia, and urinary tract infections. Characteristically, Klebsiella spp. produce large mucoid colonies because of the synthesis of large amounts of capsular polysaccharide (CPS). The LPS molecule is a virulence determinant in Klebsiella pneumoniae. In the present work from a pathogenic urine sample Klebsiella pneumoniae was obtained, the lipopolysaccharide extracted and completely purified by gel filtration chromatography. The purity was confirmed through determination of protein concentration and carbohydrate.


Keywords: Lipopolysaccharide, Klebsiella.

Download this article as Download

How to cite this article:

Abeer Ghassan Munther Al-Agha. 2017. Extraction and Purification of Lipopolysaccharide of Klebsiella pneumoniae IsolatesInt.J.Curr.Microbiol.App.Sci. 6(8): 90-100. doi: https://doi.org/10.20546/ijcmas.2017.608.012
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

Citations
Home | Terms of Use | Copyright Form 2012-2020 Copy rights reserved IJCMAS. Powered by ThinkNEXT Technologies