National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Asparaginase (asn) is a promising candidate for the obviation of acrylamide formation in baked and fried food stuffs by pre amidohydolase of free asparagine present. In this study optimized the medium composition for asparaginase production from Ochrobactrum. intermedium using one variable at a time approach. Maximum yield of asparaginase was recorded with batch time 18 h, inoculum size 2%, pH 7.0, and temperature 40ËšC. Lactose, yeast extract, magnesium ions, asparagine were found to be best carbon, nitrogen, mineral ions and inducer respectively for asparaginase production. An overall 5.045 (13.956±0.07 IU/ ml) fold increased as compared to initial un optimized activity of 2.766 IU/ml was obtained. These results suggested that selected parameter can be promisingly enhanced the asparaginase production. The further attempts are underway for validation of significant factors by statistical approach to influence asparaginase yield from the strain for acrylamide inhibition.
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