National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
There has always been a controversy in bacteriological assessment of sputum samples in diagnosing lower respiratory tract infections (LRTIs). Commonly in the Microbiological laboratory, expectorated sputum samples are microscopically examined for diagnosing LRTIs. The study was aimed to determine the diagnostic value of sputum gram’s stain and sputum culture in Lower Respiratory Tract Infections in a tertiary care hospital. Lower respiratory tract secretion (sputum) of 233 patients was cultured, identified and antimicrobial susceptibility by Kirby-Bauer disc-diffusion method was performed by standard methods. Quality of expectorated sputum samples were assessed by using Bartlett’s grading system. Among acceptable category, 141(77.05%) samples showed culture positivity. Among non-acceptable category, 42(22.92%) samples showed culture positivity. Streptococcus pneumoniae 37(20.22%) was the commonest isolated organism followed by Klebsiella pnemoniae-30(16.39%), Escherichia coli-22(12.03%), Staphylococcus aureus-21(11.47%) and Pseudomonas aeruginosa-18(09.84%). In this study, authors have recommended receiving good quality of sputum and the subsequent Gram stain and culture of sputum can provide a high diagnostic yield for clinically relevant LRTIs.
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