National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
This study aimed to evaluate the genetic diversity and identify potato cultivars by SSR markers. The genomic DNA of 50 potato cultivars was amplified with nineteen Simple Sequence Repeats (SSRs) primers. Out of nineteen primers only fifteen primers were amplified and gave polymorphic bands. Therefore, fifteen SSR primers were used in diversity analysis of 50 potato genotypes. Out of fifteen primers, four primers were found to be monomorphic and rests were polymorphic. 11 SSR primers pairs which were amplified polymorphic band that generated 34 polymorphic bands. Most of the primers detected more than one loci and observed to be polymorphic thus able to differentiate different genotypes. The dendrograms generated by cluster analysis distinguished the cultivars genetically. The PIC values demonstrated the high information content of the primers used and 50 potato varieties were identified based on SSR primer pairs. Thus, by means SSR markers the genetic diversity was assessed and the 50 potato genotypes analyzed in this study were identified.
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