Thymidine kinase (EC 2.7.1.21) is the key enzyme in the pyrimidine salvage pathway catalyzing the transfer of the phosphate from ATP to thymidine to produce dTMP. Several analogue of viral TK has been studied for inhibiting the function of TK in human viruses, but there is no any analogue for WSSV TK. Hence this study has undertaken to derive the mechanism of WSSV TK structurally based functions, in this Study Total RNA was isolated, WSSV TK gene was amplified from synthesized C-DNA, initially TK gene was cloned in PTZ T tail vector, subcloned I pRSET-B vector and expressed for further study, purified product from pRSET-B vector was sequenced and translated, blast result shown the match from Leishmania donovani TK protein. Furthermore physio-chemical parameter of WSSV TK protein was analyzed, and active sites in 3Dstructure of WSSV TK protein was studied, which will lead towards to design small inhibitory analogue of WSSV TK.

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