National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The present study suggest that the Chilika Lake isolate i.e., Bacillus sp. may act as a potent strain for alkaline α- amylase production. In case of parent strain the enzyme production was higher with banana peels as the substrate; in case of mutant, potato peels used as substrate. Optimization of the fermentation parameters and the use of suitable carbon, nitrogen and metal chloride supplements resulted in increase in the enzyme yield. The enzyme production was significantly high at optimum temperature which was found to be 37 °C and the optimum inoculums size 2%. Maximum production was found when the medium is supplemented with starch as carbon source, yeast extract as nitrogen source and NaCl as mineral chloride. The enzyme was found to be active over a wide range of pH and showed the optimum activity at pH 11. This isolate both wild and mutant could thus be industrially exploited for the synthesis of alkaline α- amylase which have immense industrial application.
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