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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 6, Issue:7, July, 2017

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(7): 2611-2615
DOI: https://doi.org/10.20546/ijcmas.2017.607.308


Antagonistic Activity and Shelf Life Study of Trichoderma harzianum (Rifai)
D.Srinivasa Reddy*, Naveena Haritha and M. Pushpa Latha
Bio-Control Laboratory, Horticulture College and Research Institute, DRYSRHU, Ananthrajupeta-516105, Koduru, Y.S.R. District, Andhra Pradesh, India
*Corresponding author
Abstract:

Trichoderma harzianum, a biological control agent seems to be suitable for management of plant pathogens. In dual culture, antagonistic activity of T. harzianum was effective against Sclerotium rolfsii showing 66.60 percent inhibition, Fusarium solani with 56.70 percent inhibition and 30.90 percent inhibition against Fusarium oxysporum. The formulation study states that it can be also promoted in liquid formulation which has higher shelf life and stress protection. Paraffin oil, soy bean oil, combination of paraffin oil and glycerol (1:1), paraffin oil and soybean oil (1:1), soya bean oil and glycerol(1:1), were used. Absence of colonies of Trichoderma harzianum was observed in soybean oil and glycerol (1:1) at 42nd day, whereas on 49th day Trichoderma showed nil growth in paraffin oil and glycerol (1:1). On 56th day paraffin oil and soybean oil showed nil growth of Trichoderma. Paraffin oil showed higher activity of Trichoderma harzianum with 20 x107cfu/ml followed by soybean oil with 2.1x106 cfu/ml at 56th day.


Keywords: Trichoderma harzianum, Antagonistic, Shelf life, Oils, Pathogens.

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How to cite this article:

Srinivasa Reddy, D., Naveena Haritha and Pushpa Latha, M. 2017. Antagonistic Activity and Shelf Life Study of Trichoderma harzianum (Rifai).Int.J.Curr.Microbiol.App.Sci. 6(7): 2611-2615. doi: https://doi.org/10.20546/ijcmas.2017.607.308
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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