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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 5, Issue:4, April, 2016

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(4): 218-225
DOI: http://dx.doi.org/10.20546/ijcmas.2016.504.027

Determination of L-Glutaminase Activity by Some Bacterial Species
Gulbahar F. Karim1 and Karkaz M. Thalij2*
1College of Nursing–University of Kirkuk-Iraq
2Department of Food Sciences-University of Tikrit-Iraq
*Corresponding author

Out of 200 clinical samples, 178(89%) bacterial isolates were recovered. Based on, cultural, morphological, and biochemical testes, there were 87 (48.88%) isolates of Gram positive cocci belong to the genus Staphylococcus, including, 63(35.39%) and 24(13.48%) isolates of Staph aureus and Staph epidermidis respectively. Whereas the 91(51.12%) remainder isolates were belong to the family Enterobacteriaceae and distributed as 56(31.46%), 23(12.92%) and, 12(6.74%) isolates of E. coli, Pseudomonas aeruginosa and Citrobacter diversus respectively. All the bacterial isolates were screened for L-glutaminase enzyme activityusing rapid plate assay. Twenty six (14.61%) isolates were found to be L-glutaminase producers. The zone index was calculated for all L-glutaminase producing samples which are ranged from (3.0-0.25). The maximum zone index was recorded by Pseudomonas aerogenosa. The enzymatic activity were ranged from(18.5-6.9)IU/ml. However the maximum activity was recorded for E. coli. No.7, Hence this isolate was selected to produce large scale from the L-glutaminase enzyme for further investigations.

Keywords: L-glutaminase, Production,E. coli and Pseudomonas aerogenosa

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How to cite this article:

Gulbahar F. Karim and Karkaz M. Thalij. 2016. Determination of L-Glutaminase Activity by Some Bacterial SpeciesInt.J.Curr.Microbiol.App.Sci. 5(4): 218-225. doi: http://dx.doi.org/10.20546/ijcmas.2016.504.027
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