Out of 200 clinical samples, 178(89%) bacterial isolates were recovered. Based on, cultural, morphological, and biochemical testes, there were 87 (48.88%) isolates of Gram positive cocci belong to the genus Staphylococcus, including, 63(35.39%) and 24(13.48%) isolates of Staph aureus and Staph epidermidis respectively. Whereas the 91(51.12%) remainder isolates were belong to the family Enterobacteriaceae and distributed as 56(31.46%), 23(12.92%) and, 12(6.74%) isolates of E. coli, Pseudomonas aeruginosa and Citrobacter diversus respectively. All the bacterial isolates were screened for L-glutaminase enzyme activityusing rapid plate assay. Twenty six (14.61%) isolates were found to be L-glutaminase producers. The zone index was calculated for all L-glutaminase producing samples which are ranged from (3.0-0.25). The maximum zone index was recorded by Pseudomonas aerogenosa. The enzymatic activity were ranged from(18.5-6.9)IU/ml. However the maximum activity was recorded for E. coli. No.7, Hence this isolate was selected to produce large scale from the L-glutaminase enzyme for further investigations.

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