National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Enterococci mainly being commensals in human faeces are now considered as an important cause of nosocomial infections. Of the infections, the commonly observed are urinary tract infections, abdominal infections followed by bacteremia, endocarditis and meningitis rarely. Speciation of Enterococcus aids in effective management of the infections caused by them. The initial treatment for enterococcal infections has become challenging due to development of resistance. The resistance has been observed mainly against aminoglycosides due to the presence of bi-functional gene. VRE strains are mostly isolated from patients with recurrent bacteremia, endovascular infections leading to increase deaths in the patients. To isolate and speciate enterococcal isolates from clinical samples, study the antibiotic susceptibility pattern and the genotype associated with the aminoglycoside and vancomycin resistance. Enterococcus isolates obtained from various sections were characterized by conventional phenotypic methods. The antibiotic susceptibility was studied by disc diffusion method. The resistant strains were further confirmed by MIC using automated methods following which the genotypic analysis was done. The incidence of Enterococci was 2.5% in which around 86.4% were obtained as pure isolates. Among the species E. faecalis was found to be the maximum. The urinary isolates exhibited sensitivity of around 92.3% to nitrofurantoin and among the non - urinary isolates the maximum sensitivity was for linezolid. The molecular study showed van A to be most common gene with vancomycin resistance (71.42%), and bifunctional gene among the aminoglycoside resistance (96%). Enterococcus faecalis was the commonest species isolated. The maximum number of isolates was obtained from urinary samples. The increase incidence of resistance gene to high level gentamicin could result in failure to the synergy treatment of gentamicin in combination with penicillin group of antibiotics. The emergence of vancomycin resistant strains among the clinical isolates makes treatment options more challenging.
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